Proteomics of matrix production - FRCs

Data to accompany figure S2
Proteomics of FRC-derived matrices
In vitro FRC cell line-derived matrices generated after 5 days in culture were subjected to proteomic analysis by mass spectrometry. Summary data in xls spreadsheet.

SWATH dataset uploaded to PRIDE repository ID=PXD015816



Abstract

Lymph nodes (LNs) work as filtering organs, constantly sampling peripheral cues. This is facilitated by the conduit network, a parenchymal tubular-like structure formed of bundles of aligned extracellular matrix (ECM) fibrils ensheathed by fibroblastic reticular cells (FRCs). LNs undergo 5-fold expansion with every adaptive immune response and yet these ECM-rich structures are not permanently damaged. Whether conduit integrity and filtering functions are affected during cycles of LN expansion and resolution is not known. Here we show that the conduit structure is disrupted during acute LN expansion but FRC-FRC contacts remain intact. In homeostasis, polarised FRCs adhere to the underlying substrate to deposit ECM ba-solaterally. ECM production by FRCs is regulated by the C-type lectin CLEC-2, expressed by dendritic cells (DCs), at transcriptional and secretory levels. Inflamed LNs maintain conduit size-exclusion, but flow becomes leaky, which allows soluble antigens to reach more antigen-presenting cells. We show how dynamic communication between peripheral tissues and LNs changes during immune responses, and describe a mechanism that enables LNs to prevent inflammation-induced fibrosis.

Highlights

FRCs use polarized microtubule networks to guide matrix deposition

CLEC-2/PDPN controls matrix production at transcriptional and post-transcriptional levels

FRCs halt matrix production and decouple from conduits during acute LN expansion

Conduits leak soluble antigen during acute LN expansion