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An oral pH-responsive Streptococcus agalactiae vaccine formulation provides protective immunity to pathogen challenge in tilapia

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posted on 2022-10-26, 08:45 authored by Sudax MurdanSudax Murdan, Nguyen Ngoc Phuoc, Abdul BasitAbdul Basit, Ruth N. Zadoks, Tharangani Herath, Shazia Bashir

Data related to the Table and figures in the publication (in title)


Table 1 shows Particle size measured by dynamic light scattering (mean±SD), n= 12 

Figure 5 shows   the Change in particle size (measured by dynamic light scattering) upon exposure to 0.1M HCl, which is simulating the acidic environment in a fasted tilapia stomach. Means are shown. Error bars reflect the SD. N=12


Fig 6 shows the pH measured in the different sections of the gastro- intestinal tract of fasted and fed tilapia (mean ± SD), N=5


Fig. 7 Shows the Cumulative percentage mortality in the 3 groups following oral gavage with: (ai) PBS in the negative control group; (aii) Blank Eudragit microparticles; (b) GBS-loaded Eudragit microparticles, and challenge by immersion using a homologous strain of Streptococus agalatiae sequence type on day 21 post-gavage. Error bars represent SD from 4 replicate tanks (25 fish per tank) for each of 3 treatment groups.

The data are shown in Excel files.  Data was collected using dynamic light scattering (Table 1, Figure 5), by measuring pH using a probe (Figure 6), and by counting the numbers of fish which survived the pathogen challenge with time. 


An internal UCL award funded through Research England’s ‘QR Global Challenges Research Fund’ and the authors’ universities. Furthermore, UCL, Hue University (, The University of Sydney ( and Harper Adams University ( funded SM’s, NNP’s, RZ’s and TH’s time, respectively, on the project. We also thank the Hue University Strong Research Group (NCM.DHH.2022.05) for staff and facilities support.