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Development of an in vitro media perfusion model of Leishmania major macrophage infection

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posted on 2019-05-09, 13:57 authored by Alec O'Keeffe, Lauren Hyndman, Sean McGinty, Alaa Riezk, Sudax MurdanSudax Murdan, Simon L Croft
Influence of the rate of flow (of culture medium) on infection of macrophage cells by L. major promastigotes

Phagocytosis of fluorescent latex beads and macropinocytosis of pHrodo Red dextran by infected macrophages (PEMs) under static or flow culture conditions

Funding

BB/J014567/1

EP/M506539/1

EPM508056/1

The Dr Hadwen Trust (Alan & Kathie Stross Summer Fellowship)

Kirkstall Ltd (financial donation)

cara (https://www.cara.ngo/who-we-are/partners-and-supporters/cara-scholars-at-risk-uk-universities-network/ )

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    University College London

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    Keywords

    in vitro media perfusion model of Leishmania major macrophage infectionLeishmaniasis, cutaneousphagocytosismacropinocytosis uptakeinfected cellspromastigotesamastigotesculture conditionsStaticflowdevelopmentperfusionmodel analysisinfection ratemathematical modellingComputational modellingpathogen infectivityIn Vitro Assaysfluid flowMedical Parasitology

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