NHS FTIR

Fourier transform infrared (FTIR) spectroscopy (PerkinElmer Spectrum 100 FT-IR) was also performed to confirm conjugation of antibody to polymeric nanoparticles. The background scan and the sample scan consisted of 8 scan times. A range of antibody concentrations (1μL, 2μL, 4μL) was tested and added before incubation overnight. For this test, the samples were lyophilised, then dispersed on the crystal light path and compressed to obtain respective spectra in the wavelength region of 4000 to 650cm^-1.

The data above are Fourier Transform Infrared (FTIR) spectroscopy data from n-hydroxysuccinimide (NHS)