Supplementary Data: Multimodal imaging reveals multiscale mechanical interplay in vertebral endplate microarchitecture during intervertebral disc loading

Data related to the publication "Multimodal imaging reveals multiscale mechanical interplay in vertebral endplate microarchitecture during intervertebral disc loading", Parmenter et al., 2024, https://www.biorxiv.org/content/10.1101/2024.08.19.608559v1

Full methodology described in the above publication.

Total data size = 41 GB

Data used for statistical analysis:

sCT_DVC_stats_data.csv : Data used for statistical analysis shown in Fig. 3 and Fig. 4

DIAD_stats_data.csv : Data used for statistical analysis shown in Fig. 6

WAXD processing code:

processing_folder_D-period.py : python code for measuring diffraction peak parameters

ctMapperGUI.py : python code for mapping WAXD parameters onto X-ray projection image

Example sCT and DVC dataset (sample 5):

162365_2560xy_2160z_pag100_us2p5_8bit.tif : reconstructed sCT image of sample under 1 N preload, 13 GB, recommend opening in imageJ

162366_2560xy_2160z_pag100_us2p5_8bit.tif : reconstructed sCT image of sample after 40 micron displacement, 13 GB, recommend opening in imageJ

162365_caudal_mindist4p5_mesh.inp : mesh of caudal VEP used for creating the DVC pointcloud, ABAQUS input file

162366_cranial_mindist4p5_mesh.inp : mesh of cranial VEP used for creating the DVC pointcloud, ABAQUS input file

162365_caudal_pointcloud.txt : caudal VEP pointcloud for DVC

162365_cranial_pointcloud.txt : cranial VEP pointcloud for DVC

dvc_162365_caudal.disp : DVC displacement results for caudal VEP, output from iDVC executable, can visualise in Matlab

dvc_162365_cranial.disp : DVC displacement results for caudal VEP, output from iDVC executable, can visualise in Matlab

sample5_caudal_step1.csv : strain data for caudal endplate generated from disp file

sample5_caudal_step1.csv : strain data for cranial endplate generated from disp file

sample5_force_readout.xlsx : force, displacement, time data readout from the mechanical testing rig during the experiment

sample5BinaryROIs.zip : binary images of regions used for microarchitecture analysis

sample5ROImsvalues.xlsx : microarchitecture values for regions of interest

Example correlative sCT and WAXD dataset (sample 16):

17565_2560xy_2160z_us1_8bit.tif : reconstructed sCT image, 13 GB, recommend opening in imageJ

17565_BinaryROI_tifs : binary image regions used for microarchitecture analysis

17567 002 dperiod map.png : 002 d-period mapped on X-ray projection

17567 002 width map.png : 002 peak width mapped on X-ray projection

17567_310_Peak_D-period_data.txt : 310 d-period for each beampath through the sample

17567_310_Peak_width_data.txt : 310 peak width for each beampath through the sample

17567_D-period_data_002.txt : 002 d-period for each beampath through the sample

17567_Width_data_002.txt : 002 peak width for each beampath through the sample

k11-17567.nxs : raw diffraction data (part 1), recommend opening in DAWN (both parts required)

k11-17567.zip : raw diffraction data (part 2), recommend opening in DAWN (both parts required)

k11-17567_processed_220722_184414.nxs : processed diffraction data, recommend opening in DAWN