Supplementary Fig. 5c & 6b | Western blots of FUS and TARDBP in iPSC lysates

This item is part of the Figshare Project:
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution

From Data Availability Statement for the forthcoming paper in Nature Communications entitled:

Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS

"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.

[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."

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[Item specific description:]

This data belong to Supplementary Fig. 5 and 6.

Western blots in iPSC lines with various FUS and TARDBP M337V mutations for FUS and TDP-43 protein expression.

The assembled western blots are presented in the PPTX file.

The fluorescent signal were scanned on a Odyssey CLx scanner (LI-COR) and exported as Image Studio ZIP archives. More information about the Image Studio software can be obtained here: https://www.licorbio.com/image-studio

The raw images are given as TIF files, thumbnails as PNG, and aquisition meta information as TXT files within the exported archives.