Validation of mouse model: qPCR and Western blots probing for Tor1a-torsinA

<p><strong>Model validation dataset associated with </strong><em><strong>Pocratsky et al 2023, Science Translational Medicine</strong></em></p> <p><br></p> <p>Data generated from postnatal day 18 littermate control (Cdx2::wt;Tor1a-wt/frt) and spinal <em>Tor1a </em>d-cko mice (Cdx2::FlpO;Tor1a-frt/frt) that develop early onset generalised torsional dystonia. Whole tissues were snap frozen and processed for expression of <em>Tor1a </em>and torsinA protein. Raw images of Western blots provided</p> <p><br></p> <p><strong>Validation_qPCR.xlsx </strong>- dataset: <em>Tor1a </em>expression in brains and lumbar spinal cords</p> <p><br></p> <p><strong>Validation_Westerns.xlsx</strong> - dataset: torsinA expression in brains and lumbar spinal cords</p> <p><br></p> <p><strong>Western_metadata</strong>: details for attached images of blots</p> <p><br></p> <p>Full methodological detail provided in manuscript</p>