Validation of mouse model: qPCR and Western blots probing for Tor1a-torsinA
dataset
posted on 2023-03-27, 14:18 authored by Amanda PocratskyAmanda Pocratsky, Filipe Do Nascimento Xavier FernandesFilipe Do Nascimento Xavier Fernandes, Gorkem OzyurtGorkem Ozyurt, Ian WhiteIan White, Roisin Sullivan, Benjamin O'CallaghanBenjamin O'Callaghan, Calvin Smith, Sunaina SuranaSunaina Surana, Marco BeatoMarco Beato, Rob BrownstoneRob BrownstoneModel validation dataset associated with Pocratsky et al 2023, Science Translational Medicine
Data generated from postnatal day 18 littermate control (Cdx2::wt;Tor1a-wt/frt) and spinal Tor1a d-cko mice (Cdx2::FlpO;Tor1a-frt/frt) that develop early onset generalised torsional dystonia. Whole tissues were snap frozen and processed for expression of Tor1a and torsinA protein. Raw images of Western blots provided
Validation_qPCR.xlsx - dataset: Tor1a expression in brains and lumbar spinal cords
Validation_Westerns.xlsx - dataset: torsinA expression in brains and lumbar spinal cords
Western_metadata: details for attached images of blots
Full methodological detail provided in manuscript
Funding
The contribution of spinal circuit dysfunction to movement disorganisation in dystonia
Beyond The Basal Ganglia: Spinal Circuit Dysfunction As A Final Pathway For Movement Disorganisation In Tor1a Dystonia
Medical Research Council
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