.TIF
Composite-Fig1B,C,D.tif (1.82 GB)
.TIF
Composite-Fig3A.tif (1.25 GB)
.TIF
Composite-Fig3B.tif (1.51 GB)
.TIF
Composite-Fig3C.tif (2.04 GB)
.TIF
Composite-Fig3D.tif (2.87 GB)
.TIF
Composite-4A.tif (3.65 GB)
.TIF
Composite-Fig4B.tif (2.98 GB)
.TIF
Composite-Fig4C.tif (1.34 GB)
.TIF
Composite-Fig5A.tif (2.82 GB)
.TIF
Composite-Fig5B.tif (618.11 MB)
.TIF
Composite-Fig5C.tif (3.34 GB)
IMAGE
Composite-Fig5D.tif (4.31 GB)
.TIF
Composite-Fig6A.tif (3.78 GB)
IMAGE
Composite-Fig6C.tif (4.2 GB)
ARCHIVE
Composite-Fig1A.7z (477.11 MB)
ARCHIVE
Composite-Fig6B.7z (1.4 GB)
1/0
3D urine-tolerant human urothelial model to study urinary tract infection
3D confocal images of:
Figure1: 3D-UHU model
Figure3: UPK1,II,III, and Chondroitin sulphate
Figure4: CK8, CK13, and CK20
Figure5: E-cadherin, ZO-1, Claudin1, and Claudin3
Figure6: TLR2, TLR4, and TLR5
3D-UHU grown on filter membranes were Immunostained then mounted with ProLong Glass antifade mountant (Invitrogen) and imaged on a Leica SP8 microscope. Images were acquired using 63x magnification with 0.3 µm Z step size giving 50% image overlap required for 3D image reconstruction. Images were processed using LAS X software version 3.5.7.
